蛋白质双水相液液平衡的热力学模型与研究进展

Thermodynamic Model and Development of Liquid Balance of Protein Aqueous Two-phase System

双水相体系由于性质温和、操作方便、分离效率高,以及不会导致被分离物质的破坏和失活等优点,被广泛应用于生物大分子物质的分离和纯化。目前主要应用的双水相体系有高聚物-高聚物体系、高聚物-电解质体系和近年来刚发展起来的胶束双水相体系。针对此3种不同类型的分离体系,分析了蛋白质在其中分配系数的热力学模型,希望对进一步研究有所帮助和启发。

The aqueous two-phase system has been applied to the separation of biomolecules he cause of its gentle character, easy operation and high separating efficiency. The systems that are being used may include the poly poly system, the poly-electrolyte system and the newly-devel oped micellar system. In this paper, we analyzed the thermodynamic models for the partition cal culating coefficients of protein in each system, hoping that is will be of help for further research.