半夏的病毒危害及脱毒快繁技术研究

Virus damage to Pinellia ternata and its rapid-proliferation technique for virus-free seedlings

目的确定病毒病对半夏植株生长的危害,通过组织培养手段对病毒进行脱毒技术研究.方法采取高温钝化结合茎尖培养的方法,用多种激素诱导茎尖成苗、长根及增殖等.结果用6-BA 1.0～2.0 mg/L、NAA 0.2mg/L诱导成苗可有效地脱去病毒;6-BA 2.0 mg/L附加适量的NAA和GA3可促进珠芽增殖及试管苗的生长速度;IAA 0.2～0.5 mg/L可促进半夏根系的发育;6-BA 2.0 mg/L、GA3 0.2 mg/L配以适当质量浓度的2,4-D和NAA可促进叶片愈伤组织形成丛生苗.结论对半夏病毒病的脱毒率达到80%,脱毒植株叶片的内部结构排列有序、清晰可见.

Objective To find out virus damage to plantlet growth of Pinellia ternata ancl study its rapid-proliferation technique for virus-free seedlings. Methods Combining stem tip culture with high temperature macle virus blunt, the tips were induced to seedling, rooting, ancl multiplicating by several hormons, then seedlings to virus-free by 6-BA 1.0-2.0 rag/L, NAA 0. 2 mg/L. Results Adding 6-BA 2.0 mg/L with a proper quantity of NAA ancl GA3, shoots ancl growth speed of tube seedlings could be accelerated. P. ternata root could be developed by IAA 0.2--0. 5 mg/L. Leaf callus forming cluster of seedlings could be enhanced by 6-BA 2.0 mg/L ancl GA3 0. 2 mg/L combined with a proper concentration of 2, 4-D ancl NAA. Conclusion The result shows virus-free seedling rate of P. ternata is up to 80% ancl the internal structure arrangement of virus-free leaves is in order ancl clear.