重组病毒巨噬细胞炎性蛋白对HIV-1_(SF1)的体外抑制作用

Recombinant Viral Macrophage Inflammation inhibit HIV-1SF1 In Vivo

目的:研究重组病毒巨噬细胞炎性蛋白(rvMIP)体外的抗HIV-1SF1作用效果和机制。方法:分别在HIV-1SF1接种前后将敏感的MT-4细胞系与rvMIP作用,检测MT-4细胞系病变情况和培养上清的P24抗原水平,用有限稀释PCR法测定前病毒DNA水平。结果:预先用rvMIP处理过的细胞,病毒感染后细胞病变程度很轻,培养上清的P24抗原和前病毒水平明显低于对照组,P24抗原抑制率可达90%;先感染病毒再用rvMIP处理组病毒P24抗原水平和细胞内前病毒DNA水平也显著降低,但降低程度不如先用rvMIP预处理组。结论:rvMIP能阻止病毒进入靶细胞,同时可能也通过某种机制抑制已感染细胞HIV病毒的复制。

Objective： To investigate the inhibiting effects and mechanism of recombinant viral macrophage inflammation protein rvMIP against HIV-1SF1 out of the body. Methods： The sensible MT-4 cells were cultured with the rvMIP before and after being immunized with HIV-1SF1. The pathological changes of MT-4 cells were observed after immunization and the P24 antigen in the culture supernatant was determined as well as HIV-1SF1 proviral DNA level within cells was tested by using limited diluting methods. Results： The cells pretreated with rvMIP only had little pathological changes. The P24 antigen level in supernatant and the proviral DNA level decreased markedly, with a highest P24 antigen inhibitory rate of 90%. The cells treated with rvMIP after infection also had a decrease of P24 antigen and proviral DNA level but both of all were less than pretreated cells. Conclusion： The main mechanism of rvMIP is to prevent virus＇entry .Meanwhile,it may also depress the replication of infected cell HIV through a kind of mechanism.