一种可用于PCR分析的水稻DNA简易提取法

A Simplified Rice DNA Extraction Protocol for PCR Analysis

以水稻黄化苗为材料,用NaOH溶液抽提DNA,对其用于基于PCR技术的DNA标记中的效果进行了分析。结果发现,用0.5mol/LNaOH对黄化苗进行直接处理,并用等量1mol/LTrisHCl进行稀释、中和,离心所得的上清液即可直接用于各种PCR扩增和随后的DNA标记鉴别,包括转基因PCR片段检测、微卫星标记多态性分析(琼脂糖电泳法或毛细管电泳法),用这种方法提取的DNA可以在短期内保存,在PCR分析中其效果与用常规CTAB法提取的DNA相当。

A simple protocol was established for DNA extraction by using rice etiolated seedlings. By using this method, rice DNA was directly extracted in 0.5 mol/L NaOH solution in an eppendorf tube. Results of comparative PCR analyses and electrophoresis showed that DNA extracted by using this method was as good and useful as that isolated by using standard CTAB method.