单核细胞趋化蛋白-1在子宫内膜异位症患者血清及腹腔液中的变化

Changes of monocyte chemoattractant protein-1 levels in serum and abdominal cavity fluid in women with endometriosis

目的:检测子宫内膜异位症(EM)患者与正常妇女血清及腹腔液中单核细胞趋化蛋白-1(MCP-1)水平的异同,探讨其与子宫内膜异位症发病的关系。方法:子宫内膜异位症患者52名作为研究组,正常妇女45名作为对照组,采用双抗体夹心酶联免疫吸附技术(Sandw ichELISA)检测两组妇女血清及腹腔液中MCP-1水平。结果:血清MCP-1水平两组分别为:EM组(207±76)pg/m l,正常妇女组(148±56)pg/m l,两组间比较差别有极显著意义(P<0.01)。腹腔液MCP-1水平两组分别为:EM组(292土98)pg/m l,正常妇女组(164±65)pg/m l,两组间比较差别亦有极显著意义(P<0.01)。相关性分析发现:EM组血清与腹腔液MCP-1呈正相关(r=0.471,P=0.002),正常妇女组血清与腹腔液MCP-1无明显相关性。结论:EM患者血清与腹腔液MCP-1水平均显著升高,MCP-1是EM发生的关键因子之一。

Objective： To investigate the relationship of monocyte chemoattractant protein - 1 （ MCP - 1 ） to endometriosis （EM） Methods： Levels of MCP - 1 in serum and in abdominal cavity fluid were determined by a solid quantitative sandwich enzyme immunoassay technique （Sandwich ELISA） both in EM group and in controls, and relationship of MCP- 1 levels in serum and in abdominal cavity fluid were analyzed. Results： Serum concentrations of MCP - 1 were increasd （P 〈 0. 01 ） in EM group （207 ± 76） pg/ml than that of controls （ 148 ±56） pg/L Concentrations of MCP - 1 in abdominal cavity fluid were increasd （P 〈0. 01 ） in EM group （292 ± 98） pg/ml than that in controls （164 ± 65） pg/ml. In EM, nonparametric correlations analysis demonstrated that concentrations of serum MCP - 1 were positively correlated with those in abdominal cavity fluid （r =0. 471, P =0. 002）, but such a correlation did not be found in controls. Conclusion： Serum levels of MCP - 1 in EM increase significantly and higher concentrations of MCP - 1 may be partly responsible for EM in a special way.