五味子酮对β-淀粉样蛋白诱导大鼠海马神经元内游离钙离子浓度变化的影响

Effect of Schisandrone on [Ca^(2+)]_i induced by amyloid-beta protein in cultured hippocampal neurons

目的观察中药五味子酮对β-淀粉样蛋白(Aβ25~35)诱导的大鼠海马神经元内游离钙离子浓度([Ca2+]i)变化的影响。方法采用大鼠海马神经元原代培养技术,运用双激发波荧光指示剂Fura-2/AM标记胞内相对[Ca2+]i的变化并进行比较。结果1μmol/L Aβ25~35作用于海马神经元48 h后,[Ca2+]i为0.5951±0.0311,明显高于空白对照组的0.3617±0.0197(P<0.05);1μmol/L Aβ25~35和100μmol/L五味子酮共同作用于海马神经元48 h后,[Ca2+]i为0.4168±0.0468,仍明显高于空白对照组(P<0.05),但较单纯Aβ25~35作用后明显下降(P<0.05)。结论中药五味子酮可以通过抑制Aβ诱导的神经元[Ca2+]i增加,维持细胞内钙稳态,对神经元有一定的保护作用。

Objective To observe the effect of Sehisandrone on [Ca^2＋]i induced by amyloid-beta protein in cultured hippoeampal neurons of SD rats. Methods The hippoeampal neurons of one-day-old SD rats primarily cultured for seven days were randomly placed in A,B and C dishes. Then the neurons were treated with 1μmol/L Aβ25～35 or 1μmol/L Aβ25～35 and 100 μmol/L Sehisandrone or with B27 only for two days. On the ninth day, all neurons of every dish were marked with Fura-2/AM, and the well-marked and dispersed ones were selected for detection and analysed by Metermorph and Metaflour software. The ratio of 340 nm to 380 nm represented the concentration of intraeellular free calcium. Results The ratios of neurons in dish A, B and C were 0. 5951 ±0. 0311,0. 4168±0. 0468 and 0. 3617±0. 0197 respectively. The ratios of neurons in dish B treated by 1 μmol/L Aβ25～35 and 100 μmol/L Sehisandrone were higher than the one in dish C treated by B27 only （P〈0.05）, but was lower than the one in dish A treated by Aβ25～35 （P〈0.05）. Conclusions Sehisandrone can maintain intraeellular calcium homeostasis through inhibition of increase of [Ca^2＋]i induced by beta-amyloid protein, so as to provide protection of the neurons. （Shanghai Med J, 2005,28 ： 790-792）