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两种原发大肠肿瘤细胞株蛋白质表达谱差异初步分析

Preliminary analysis of differentially expressed protein profiles in two primary colonic tumor cell lines
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摘要 目的应用双向电泳技术分析大肠侧向发育型肿瘤(LST)细胞株和结肠肿瘤SW480细胞株间的蛋白质表达谱差异。方法以双向电泳技术分离两种细胞株总蛋白质,银染显色,进行差异蛋白质分析。结果LST和SW480细胞株双向电泳图谱蛋白质点数分别为1285±51和1184±47,共获得96±7的蛋白质差异点,其中50±6个点仅在LST细胞株中表达或表达明显增强,47±5个点仅在SW480细胞中表达或表达明显增强。结论大肠LST细胞株与SW480细胞株的蛋白质表达谱存在一定的差异,对这些蛋白质点进行鉴定将为研究大肠LST特殊性生物学行为提供一定的线索。 Objective To analyze the differences in protein profile between laterally spreading tumor （LST） cell line and SW480 cell line using two-dimensional electrophoresis （2-DE）. Methods 2-DE was employed to isolate the total proteins of the two cell lines. The gels were stained with silver, and the differential protein expressions were analyzed with Melanine 3 software. Results The protein spots of the two cell lines were 1285±51（LST） and 1184±47（SW480） respectively,containing 96±7 differential protein spots with 50±6 expressed or obviously increased only in LST cell line and 47±5 in SW480 cell line. Conclusion The protein profiles between LST and SW480 cell lins are different and further analysis on the different interested spots is required to acquire the biology-associated proteins specific LST.
作者 童华生 张亚历 姜泊 赖卓胜
机构地区 南方医科大学南方医院消化内科研究所
出处 《解放军医学杂志》 CAS CSCD 北大核心 2005年第10期887-889,共3页 Medical Journal of Chinese People's Liberation Army
关键词 双向电泳 结直肠肿瘤 蛋白质组学 two-dimensional electrophoresis colorectal neoplasms proteomics
分类号 R735 [医药卫生—肿瘤] R341 [医药卫生—基础医学]
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参考文献15

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解放军医学杂志
2005年 第10期

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