转录因子Sp1在胃癌中的表达及其与预后的关系

Transcription factor Sp1 expression in gastric cancer and its prognostic value

目的研究转录因子Sp1在胃癌及正常胃黏膜组织中的表达特征,并探讨其表达对胃癌患者预后的影响。方法用免疫组织化学方法,检测65例胃癌原发灶及40例因胃良性病变行胃部分切除标本中的正常胃黏膜组织Sp1表达情况,观察其表达特性并研究该因子表达与患者长期生存率的关系。结果所测正常胃黏膜组织Sp1表达阳性率仅为12.5%(5/40),且均表达于正常胃腺的颈部,在成熟细胞较多的胃腺体底部未见有表达。与之相反,Sp1在胃癌组织中则有较高表达率,为53.8%(35/65)。肿瘤组织中Sp1蛋白呈无表达、弱表达及强表达患者的平均生存期分别为1700d、1560d及1026d,彼此间差异有统计学意义(P=0.036)。Sp1蛋白表达与肿瘤侵袭深度及TNM分期密切相关(P=0.001,P=0.026),而与淋巴结转移数目及Lauren分型无明显相关(P=0.306,P=0.667)。结论在正常胃黏膜和胃癌组织中,Sp1蛋白表达具有不同的分布特征。Sp1可作为判断胃癌患者预后的一个独立指标,并可能通过除淋巴结转移以外的其他机制促进肿瘤的侵袭和发展。

Objective To investigate the expression of transcription factor Sp1 in human gastric cancer tissues and normal gastric mucosa, and its prognostic significance. Methods By using immunohistochemistry, we studied the Sp1 expression patterns in 65 cases of gastric cancer with various clinico-pathologic characteristics, and 40 normal gastric mucosa specimens obtained from patients who underwent partial gastreetomy for benign gastric diseases. The significance of Sp1 expression on the survival of patients was evaluated. Results The expression rate of Sp1 in normal gastric mueosa was 12.5% （5/ 40）. The positively stained glandular cells were mainly limited to those in the neck region. Cells at the basal portion of the gland were essentially negative. In sharp contrast, Sp1 expression rate in gastric cancer lesions was 53.8% （35/65）. The medium survival time in patients who had a tumor with negative, weak and strong Sp1 expression was 1700, 1560 and 1026 days, respectively （P = 0. 036）. Sp1 protein expression was closely related to the depth of tumor invasion and TNM stage （ P = 0. 001, P = 0. 026 ） , but not related to the number of metastatic lymph nodes and Lauren＇s classification （ P = 0.306 , P = 0.667 ） . Conclusion Normal and malignant gastric tissues have unique Sp1 expression patterns. Sp1 might be served as an independent prognostic factor.