西南地区茶生柱锈重寄生菌的分离与鉴定

Isolation and identification of Cronartium ribicola mycoparasites in southwest China

华山松疱锈病是华山松Pinus armandi最严重的病害之一。为了利用重寄生菌对华山松疱锈病进行生物防治,对其病原菌茶生柱锈Cronartium ribicola的重寄生菌进行了分离和鉴定。依据柯赫氏三原则对分离得到的真菌进行鉴定,得到茶生柱锈的重寄生菌链格孢Alternariaspp.,镰刀菌Fusariumspp.,棒束孢Isariasp.,拟盘多毛孢Pestalotiopsissp.,木霉Trichodermaspp.及粉红单端孢Trichothecium roseum。经显微和超显微观察发现接种重寄生菌后锈孢子受到不同程度的破坏,且不同的重寄生菌对锈孢子有不同的作用方式。

Pinus armandi blister rust is one of the most severe diseases in P. armandi forest. In order to biologically control this disease with mycoparasites, isolation and identification of mycoparasites on Cronartium ribicola were conducted. The results showed that Alternaria spp., Fusarium spp., Isarta sp., Pestalotiotiopsis sp., Trichoderma spp. and Trichothecium roseum were mycoparasites in Cronartium ribicola. Microscopic and ultra-microscopic observation of aeciospores under ESM showed that different mycoparasites had different effects on aeciospores. [Ch, 3 fig. 4 tab. 15 ref. ]