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泌乳前后家猪乳腺基因表达谱的构建以及不同猪种和不同发育时期差异表达基因的确定 被引量：2

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摘要 构建了不同猪种、不同发育时期的非标准化家猪乳腺cDNA文库,并从中获得28941条高质量ESTs(expressed sequence tags).利用序列拼接软件CAP3,这些EST序列被拼接成2212条重叠群和5642条单一序列.这些序列在经过功能注释后被聚类成6857个基因聚类,其中2072条无相应功能注释的序列被认为来自于新基因.按照标准基因词汇体系的分类标准,已经有功能注释的基因进行了聚类分析.通过比较基因表达谱,确定了几组在猪种间和猪种内不同发育时期乳腺中差异表达的基因,这些基因中某些可能与家猪的繁殖特性相关,另外则在乳汁合成、分泌和乳腺复旧过程中发挥重要的作用.这些来源于家猪乳腺特定发育时期的基因表达谱和一些功能未知的EST序列为进一步的研究提供了重要资源.
作者 苏志熙 董新姣 张兵 曾燕舞 傅衍 于军 胡松年
机构地区 浙江大学沃森基因组科学研究院 温州师范学院生命与环境科学学院
出处 《中国科学（C辑）》 CSCD 北大核心 2005年第5期462-471,共10页 Science in China(Series C)
基金 科学技术部"863"国际合作项目(批准号:2002AA229061) 中国科学院知识创新重大项目(批准号:KSCX1-01)资助
关键词 表达序列标签 表达谱 家猪 乳腺 差异表达基因 基因表达谱 发育时期 猪种 构建 EST序列
分类号 S828 [农业科学—畜牧学]
  • 相关文献

参考文献32

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共引文献47

同被引文献39

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中国科学（C辑）
2005年 第5期

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