一种优化的蜜环菌分离与纯化方法

An Optimized Isolation and Purification Method for Armillaria mellea Complexes

从分离材料的采集、蜜环菌索的室内培养条件、培养基的改良和分离技术的优化4个方面对前人的蜜环菌分离方法进行了系统的完善和改进。结果表明:采用蜜环菌生长良好的木本植物组织为分离材料,在15℃保湿的条件下培养可以得到健壮的菌索;按玉米粉¨黄豆粉¨蔗糖¨水=6¨1¨1¨26的质量比例制作的培养基不仅能满足蜜环菌生长的营养需求,而且对于其他杂菌具有明显的阻滞作用,有助于菌株纯化;分离前对菌索表面进行适当干燥可显著提高分离的成功率。

The free-disinfection isolation method of Armillaria mellea complexes was modified in 4 ways. Firstly, the tissues of trees which were well parasitized by A. mellea complexes should be selected as the materials for culturing rizhomorph, but the tissues of grasses （or bamboo）, or the soil that A. mellea complexes existed are not suitable. Secondly, the target tissues were cultured in a high humidity and temperature of 15℃, then well-grown A. mellea complexes rhizomorph can be obtained. Thirdly, the culture medium, made up of maize：soya：sucrose：water=6：1：1：26 in mass, not only can supply enough nutrition, but also can hinder the growth of the bacteria and other fungi, which was a concomitance of A. mellea complexes rhizomorph. And last, if the rhizomorph for isolation was exposed in the air for 8-10 minutes, then the infected probability by bacteria can be reduced significantly.