乳腺癌中CD105表达及相关因素分析

Expression of CD105 and significance for prognosis in breast carcinomas

目的多项免疫组化(multiple immunohistochemistry, MIHC)检测内皮因子CD105作为判断患者乳腺癌生物学行为及预后指标的可能性.方法选取2003年11月～2004年1月经河北医科大学病理科确诊为原发性乳腺癌患者石蜡包埋组织标本87例,癌旁组织标本20例,同期乳腺良性病变标本24例.以CD105单克隆抗体为标记物进行免疫组化染色,根据CD105的表达水平计测微血管密度(microvessel density, MVD).结果 CD105所标记的MVD在乳腺癌组织和乳腺良性病变以及乳腺良性病变与癌旁组织之间的表达差异均有显著性(P<0.05),癌旁组织中CD105几乎表达缺失;淋巴结转移阳性、组织分化差(Ⅲ级)、临床TNM分期晚以及VEGF表达阳性、ER、PR表达阴性的患者中,CD105的表达明显升高,差异均有显著性(P<0.05).结论 CD105主要在处于增殖状态的血管内皮细胞上表达,在标记肿瘤组织活性微血管方面特异性极高,是检测乳腺癌患者预后的重要指标.

Purpose To investigate the possibility of CD105 as a marker for prediction of prognosis in breast cancer. Methods 87 cases of breast cancers, 20 cases of adjacent tissues, 24 cases of benign tumors were obtained from December 2003 to February 2004. The paraffin-embedded blocks of all samples were stained immunohistochemically using monoclonal antibody against of CD105 followed by detection of microvessel density （MVD）. Results The MVD based on CD105 expression had statistical significance not only between breast cancers and benign tumor of breast, but also between benign tumors and normal breast tissues. CD105 was seldom expressed in normal breast tissue. High level of CD105 expression was demonstrated in the cases with metastasis of lymph node, expression of VEGF, loss of ER PR expression or late TNM stages. Conclusions CD105 is strongly expressed on proliferating vascular endothelium and specific in marking the activity microvessel of tumor. Investigating CD105 will contribute to prediction of the prognosis of breast cancer.