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用YO-PRO-1和PI联合染色定量检测细胞凋亡 被引量：4

Detection of Apoptosis by YO-PRO-1/PI Staining
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摘要 经不同浓度staurosporine处理诱导凋亡的G7细胞样品,分别用YO-PRO-1/PI和AV/PI进行荧光染色,借助流式细胞仪检测凋亡情况,将两种检测方法得到的结果进行统计学分析显示,二者有显著的相关性(r=0.9659,P<0.01),且没有显著性差异(P<0.05);另外,上述凋亡细胞样品经YO-PRO-1/PI染色后在荧光显微镜下计数凋亡细胞比例的结果与AV/PI流式细胞仪的检测结果也有显著的相关性(r=0.9903,P<0.01),且没有显著性差异(P<0.05)。以上这些结果表明,用YO-PRO-1/PI对细胞进行染色、借助流式细胞仪和荧光显微镜均能准确地检测细胞凋亡,可替代AV/PI流式细胞仪方法用于细胞凋亡的检测。 Apoptosis in G7 cells was induced by different concentrations of staurosporine. Cells were stained with YO-PRO-1/PI and AV/PI respectively and apoptosis was detected by flow cytometry. The data obtained were analysed statistically. Excellent correlation values （ r = 0. 9659, P 〈 0.01 ） and no significant difference （P 〈 0. 01 ） were found between YO-PRO-1/PI staining and AV/PI staining. In addition, similar statistics were obtained between YO-PRO-1/PI-stained fluorescence microscopy and AV/PI-stained flow cytometry. The results indicated that apoptosis could be accurately detected by YO-PRO-1/PI stained flow cytometry and fluorescence microscopy and that YO-PRO-1/PI staining could substitute for AV/PI staining in the detection of apoptosis.
作者 叶玲玲 刘红 李世崇 刘兴茂 吴本传 陈昭烈
机构地区 军事医学科学院生物工程研究所细胞工程室
出处 《中国生物工程杂志》 CAS CSCD 北大核心 2005年第10期50-53,62,共5页 China Biotechnology
基金 国家863计划资助项目(2002AA2Z344F)
关键词 YO-PRO-1 PI 联合染色定量检测 细胞凋亡 荧光显微镜 流式细胞仪 YO-PRO-1 PI Apoptosis Flow cytometry Fluorescence microscope
分类号 Q25 [生物学—细胞生物学]
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参考文献12

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二级参考文献6

共引文献2

同被引文献33

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中国生物工程杂志
2005年 第10期

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