药盒法制备^(99m)Tc-RBC的人体内稳定性及内照射剂量研究

Stability and dosimetry of ^(99m)Tc-RBC prepared by kit method in healthy volunteers

目的研究用自制RBC药盒制备的99mTcRBC人体内稳定性和内照射辐射吸收剂量。方法4名健康志愿者,分别取肘静脉血1ml,用自制的RBC药盒进行放射性锝标记,每人注射481～555MBq,给药后5min,1,2.5,4,8和24h进行全身显像,测定99mTcRBC在人体内的生物分布;计算各器官在各时间点的放射性占注入量的百分数(%ID);两种方法绘制各脏器时间放射性计数曲线,计算各脏器的滞留时间,用MIRDOSE3.0软件计算出各受照器官的内照射辐射剂量。结果99mTcRBC在人体内较稳定,血池及大血管于给药后5min至8h均可清晰显影;人体内照射辐射吸收剂量估算结果与文献值相近,按一次给药555MBq计算,各器官的吸收剂量均低于50mGy。结论用本实验室自制RBC药盒制备的99mTcRBC体内稳定性好,内照射辐射吸收剂量符合要求。

Objective To study the in vivo stability of and absorbed radiation dose from ^99mTc-RBC, prepared using RBC kit made in our laboratory. Methods Four healthy volunteers were studied with ^99mTc-RBC prepared by kit method. Whole body images were acquired at 5 min, 1, 2.5, 4, 8 and 24 hours after intravenous injection of 481-555 MBq ^99mTc-RBC. Biodistribution analysis was performed in regions of interest （ROI）. The absorbed radiation doses were calculated using the MIRDOSE3.0 analysis program and the timerelated activity curves for different organs were used for the residence time calculations. Results ^99mTc-RBC prepared by kit method was stable in vivo. The quality of blood pool imaging from 5 min to 8 h in healthy volunteers was satisfactory. The absorbed radiation dose was lower than the permissible value. Conclusion ^99mTc-RBC prepared by kit method is very suitable for clinical application.