摘要
通过杀虫活性测定,获得1株对菜青虫、小菜蛾、二化螟等多种鳞翅目害虫均有较高杀虫活性的高效苏云金芽孢杆菌Bacillus thuringiensis(B t)菌株,命名为X-2.同时根据Cry1类蛋白N端部分氨基酸序列设计了1对简并引物,以苏云金芽孢杆菌X-2菌株质粒DNA为模板,应用PCR扩增技术得到一大小为3 468 bp的DNA片段(Cry1Ab X-2).该基因编码1 155个氨基酸,相对分子质量为130 780,等电点pI=5.06.序列比较表明该基因与Cry1Ab类基因高度同源(达95(以上),该基因已在GenBank中登录,登录号为AY847289.
After the determination of insecticidal activity, one Bacillus thuringiensis strain, showing high toxic to Pieris rapae, Plutclla xylostella and Chilo suppressalis, was obtained and named Bt X-2. Degenerate primers were designed based on the N-terminal partial sequence of Cryl to amplify the corresponding DNA sequence by touchdown PCR. The amplified fragment, with the length of 3 468 bp, encoding 1 155 amino acid with Mr = 130 780 and pI =5.06. The amino acid sequence of Cry1Ab X-2 gene showed more than 95% identity to other Cry1Ab, and it was registered in GenBank (Accession No. AY847289).
出处
《华南农业大学学报》
CAS
CSCD
北大核心
2005年第4期40-42,共3页
Journal of South China Agricultural University
基金
国家973计划资助项目(2003CB114400)
国家自然科学基金资助项目(30500341)
江苏省自然科学基金重点项目(BK2005214)
江苏省农业科学院重点研究领域项目