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糖蜜草内生固氮菌IS-PCR和16S rRNA基因全序列分析 被引量：17

Molecular study of endophytic nitrogen fixing bacteria isolated from Melinis minutiflora
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摘要 采用插入序列指纹图谱分析(IS-PCR)和16S rRNA基因全序列分析,对来源于优良牧草———糖蜜草M elinism inutifloraBeauv.内生固氮菌进行研究.结果表明,糖蜜草中分离的内生固氮菌与产脂固氮螺菌模式菌株DSM1691的插入序列指纹图谱存在着差异;来源于糖蜜草的4株菌紧密地聚在一起,与GenBank中已知产脂固氮螺菌Azospirillum lipoferum的16S rRNA基因全序列有97%的序列相似性.初步确定糖蜜草中分离的内生固氮菌为一新类群的内生固氮菌. Endophytic diazotrophs isolated from molassess grass, Melinis minutiflora , were studied by insert sequence（IS）-PCR fingerprinting patterns and full length of 16S rRNA gene sequences. IS-PCR fingerprinting showed that different patterns appeared between isolates and the type strain DSM 1691 of Azospirillum lipoferum; 16S rRNA gene sequence analyses indicated that homology between isolates and known species Azospirillum lipoferum is 97%. These resuks suggested that isolates from molasses grass are possible a new group of endophytic diazotrophs closely related to Azospirillum lipoferum.
作者 彭桂香 王华荣 张国霞 侯伟 袁清华 谭志远
机构地区 华南农业大学资源环境学院 广东省植物分子育种重点实验室华南农业大学农学院
出处 《华南农业大学学报》 CAS CSCD 北大核心 2005年第4期73-76,共4页 Journal of South China Agricultural University
基金 国家自然科学基金资助项目(30300001和30470002) 华南农业大学校长基金资助项目(4100-K03113)
关键词 糖蜜草 内生固氮菌 固氮螺菌 Melinis minutiflora endophytic diazotrophs Azospirillum lipoferum
分类号 Q939.113 [生物学—微生物学]
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参考文献15

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二级参考文献26

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共引文献58

同被引文献229

引证文献17

二级引证文献139

华南农业大学学报
2005年 第4期

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