全反式维甲酸对糖尿病大鼠尿转化生长因子-β_1排泄率及肾小球病变的影响

Effect of all-trans retinoic acid on the excretion of urine TGF β_1 and glomerular lesion of diabetic rat

目的观察全反式维甲酸(all-trans-retinoic acid,atRA)对链尿佐菌素(STZ)诱导的糖尿病大鼠早期肾小球病变的保护作用。方法20只雄性SD大鼠分糖尿病维甲酸治疗组、糖尿病对照组和正常对照组。糖尿病维甲酸治疗组每天给予全反式维甲酸10 mg/kg体质量皮下注射,共7 d(n=6)。检测尿蛋白和转化生长因子-β1(TGFβ1)的排泄率、血清、尿液及肾组织一氧化氮(NO)水平和肾脏病理变化。结果糖尿病对照组尿蛋白及TGFβ1排泄率、血清、尿液及肾组织NO水平和肾小球基质比例明显高于正常对照组,全反式维甲酸则减轻了这些变化。结论全反式维甲酸具有延缓糖尿病肾小球病变进展的作用,这种作用可能与抑制TGFβ1和NO的分泌有关。

Objective To investigate the effect of all-trans retinoic acid（atRA） on the urinary TGFfβ1 excretion and glomcrular lesion of diabetic rats at early stage. Methods SD rats wcrc randomly assigned into 3 groups： atRA treated group, diabetic control group and normal control group. Strcptozotocin（STZ）-induccd early diabetic male SD rat wcrc used. The atRA treated group wcrc treated with daily subcutaneous injections atRA of 10 mg/kg for 7 days （n =6）, and then the excretion of urinary protein and TGFfβ1 and NO level of plasma, urine and renal tissue were measured, and pathological changes of their kidneys were obscrved. Results The diabetic control rats showed increased urinary excretion of protein and TGFfβ1 and NO level of plasma, urine and renal tissue and deposit of glomcrular matrix, while atRA prevented these changes. Conclusion AtRA can prevent the development of diabetic ncphropathy, which is relevant with the inhibition of secretion of TGFβ1 and NO.