蝙蝠葛酚性碱对血栓形成和血小板聚集的影响

Effect of phenolic alkaloids of Menispermum dauricum on thrombosis and platelet aggregation

目的观察蝙蝠葛酚性碱(PAMD)对血栓形成、血小板聚集的影响并研究其作用机制。方法用动静脉短路血栓形成模型观察血栓形成;比浊法测定血小板聚集度;电镜技术观察血小板超微结构变化;放射免疫法测定TXB2和6-酮基-PGF1α的水平;硝酸还原酶法测定兔血浆NO浓度。结果PAMD体内给药可剂量依赖性地抑制血栓形成及由ADP,AA和THR诱导的大鼠和兔的血小板的聚集;可显著抑制血小板超微结构的变化;能明显升高兔血管壁6-酮基-PGF1α产生量,对血小板释放的TXB2无明显影响;还可提高兔血浆NO的浓度。结论PAMD具抗血栓形成和抗血小板聚集的作用,其机制与增加血管壁PG I2含量,提高兔血浆NO的浓度有关。

Aim To observe the effect of phenolic alkaloids of Menispermum dauricum （PAMD） on thrombosis and platelet aggregation, and to explore its mechanism of action. Methods Thrombosis was observed with arteriovenous shunt thrombus model in rat; platelet aggregation was determined by Born＇ s method ; ultrastructure of platelet was observed by transmission electron microscope; TXB2 or 6-keto-PGF1α levels were assessed by radioimmunoassay; and NO was determined by colorimetric method. Results PAMD dose-dependently inhibited experimental thrombus formation, platelet aggregation induced by ADP, AA and THR in vivo and ultrastructure changes stimulated by THR; PAMD increased the generation of 6-keto-PGF1α in thoracic aortae and NO level in plasma; and had no influence on TXB2 release （ P 〉 0. 05）. Conclusion PAMD inhibited thrombosis and platelet aggregation, and its mechanism might be due to the increase of PGI2 and NO level.