西洋参茎叶总皂苷碱降解成分

Alkaline-degradation products of ginsenosides from leaves and stems of Panax quinquefolium

目的研究西洋参茎叶总皂苷碱降解成分。方法采用硅胶柱色谱并结合HPLC进行分离纯化,通过波谱分析鉴定化合物的结构。结果从西洋参茎叶总皂苷碱降解产物中分离得到9种成分,分别鉴定为:20(S)-原人参二醇(I),20(S)-达玛-25(26)-烯-3,β12,β20-三醇(II),24(R)-ocotillol(III),20(S)-原人参三醇(IV),20(S)-达玛-25(26)-烯-3,β6,α12,β20-四醇(V),达玛-20(21),24-二烯-3,β12β-二醇(V I),达玛-20(21),24-二烯-3,β6,α12β-三醇(V II),20(S),24(S)-达玛-25(26)-烯-3,β6,α12,β20,24-五醇(V III),20(S)-达玛-23-烯-25-过氧羟基-3,β6α,12,β20-四醇(IX)。结论碱降解20位S构型未改变。V,V II,V III,IX为4个新化合物,并利用2D-NMR技术对新化合物的氢和碳的化学位移进行了归属。其中I对HCT-8人结肠癌细胞具有较强的细胞毒活性。

Aim To study the alkaline-degradation products of ginsenosides from leaves and stems of Panax quirutuefolium L. Methods Isolation and purification were carried out on silica gel and HPLC; the structures of chemical constituents were elucidated by spectral analysis. Results From the alkaline- degradation products, nine compounds were identified as ： 20 （S） -protopanaxadiol （Ⅰ） , 20 （S） -dammar- 25 （26） -ene-3β,12β, 20-triol （ Ⅱ ）, 24 （ R ） -ocotillol （ Ⅲ）, 20 （ S ） -protopanaxatriol （ Ⅳ）, 20 （ S ）- dammar-25 （ 26 ）-ene-3β,6α,12β, 20-tetrol （ Ⅴ）, dammar-20 （ 21 ）, 24-diene-3β,12β-diol （ Ⅵ）, dammar-20 （ 21 ）, 24-diene-3β,6α,12β-triol （ Ⅶ）, 20 （ S ）, 24 （ S ） -dammar-25 （ 26 ） -ene-3β,6α,12β,20, 24-pentanol （ Ⅷ）, 20 （S） -dammar-23-ene-25-hydroperoxyl-3β,6α,12β,20-tetrol （ Ⅸ）. Conclusion The configuration of C20 position of ginsenosides was not changed by alkaline-degradation. The complete assignments of ^1H and 13C NMR chemical shifts of four new compounds Ⅴ, Ⅶ, Ⅷ, Ⅸ, were acquired by means of 2D NMR spectra. Compound Ⅰ showed antitumor effect on human colon carcinoma cells in vitro.