极谱氧传感仪测定SOD快速诊断先天愚型及其产前诊断的研究

Study of prenatal diagnosed rapidly of Down Syndrome using tested SOD by test instrument of polarographic oxygen sensor

应用极谱氧传感检测仪，测定红细胞中的ＳＯＤ活性来鉴定Ｄｏｗｎ＇ｓ，探索替代传统的染色体诊断方法。本文检测６２例健康人红细胞的ＳＯＤ为６９３５±４８７ｕ／ｍｌ，３２例Ｄｏｗｎ＇ｓ红细胞的ＳＯＤ为１０６０２±８２９ｕ／ｍｌ。而检测４８例健康新生儿红细胞的ＳＯＤ为５６２７±４６３ｕ／ｍｌ，３６例健康胎儿红细胞的ＳＯＤ为５７０７±４７５ｕ／ｍｌ，５例Ｄｏｗｎ＇ｓ新生儿红细胞的ＳＯＤ为８３０７±２２９ｕ／ｍｌ。研究结果显示Ｄｏｗｎ＇ｓ比健康人的红细胞ＳＯＤ高１．５倍，两者具有显著性差异。本方法检测快速，只需５０分钟，灵敏度高，操作简便，具有实用推广价值。

To explore the method of appraise Down syndrome substitute for traditional chromosome diagnose, we examined SOD activity of RBC by test instrument of polarographic oxygen sensor.We tested RBC SOD of 62 healthy people was 6935±487u/ml, 32 Down's was 10602±829 u/ml. But 48 healthy newborns were 5627±463 u/ml,36 healthy fetus were 5707±475 u/ml,5 Down's newborns were 8307±229 u/ml.The results show RBC SOD of Down's more than healthy people of 1. 5 times. There were significant differences in Down's and healthy people. This method has practical popularize value, it maniplate simply, highly sensitivity,test rapidly, only 50 minutes.