周围神经损伤与再生的可视化研究

Visualization of Regenerated Sciatic Nerve of Rat After Injury

周围神经损伤后其远近端形态改变与神经再生及功能恢复有密切联系。本试验通过硅胶管桥接大鼠坐骨神经缺损模型,对不同时间点、损伤区不同部位的坐骨神经进行连续切片,光、电镜观察摄像后,利用计算机图像重建技术,采用体素模型在SGI三维图形工作站上对大鼠坐骨神经损伤后变性、再生过程中形态结构改变进行三维重建和显示。三维图像显示新生神经纤维与正常神经纤维相比有郎飞氏结尚未形成、排列紊乱、髓鞘较薄、轴索较细、胶原纤维增生等差异。

There is close relationship between the morphologic changes of peripheral nerve after injury and its function recovery during regeneration. In our experiment, the sciatic nerve of rats was transected and bridgeconnected with silicone tube, and the images of serial slices of different time and different injury parts were taken by micro-photograph system. The volume unit model was applied to rendering the three dimensional （3D） structure of degenerative and regenerative sciatic nerve fiber and its affiliated structure after injury. The 3D images showed that node of Ranvier had not formed and its array was turbulent, both the myelin sheath and the axiscylinder were thinner, and the collagen fibres had proliferated.