沙眼衣原体套式（Nested）PCR检测研究

Detection of Chlamydia trachomatis by the nested polymerase chain reaction

本文报告沙眼衣原体（ＣＴ）的套式（ＮＥＳＴＥＤ）ＰＣＲ检测方法。本方法以ＣＴ隐匿性质粒为靶基因，外套引物采用国外学者所报告灵敏度和特异性较高的序列，内套引物自行设计。经方法学考核表明本法灵敏度和特异性极高。１４６例临检标本套式ＣＴＰＣＲ阳性检出率为３６．３％，而市售ＰＣＲ试剂盒（其引物序列与本文外套引物相同）阳性检出率仅为４．１％。前者明显高于后者（Ｐ＜０．０１）。

the nested PCR was used to detect plasmidDNA from Chlamydia trachomatis(CT).The nested PCR sensitivity for deteCTion of(CT) in comparison with that of a common PCR technique was established in a follow-up study on the dilution test.The former is higher that the latter.the plasmid-DNA of CT was detected in the cervical secvetions of 146 cases by a common PCR and the nested PCR simultaneously.The results show that the positive rate of the nested PCR was significantly higher than that of a common PCR(p<0.01).Our results suggest that the nested PCR method may be a specific,sensitive and simple that for CT.