女性生殖道三种常见病毒采用多重聚合酶链反应检测方法的探讨

Study of detective method for several common virus from female reproductive tract.

多重聚合酶链反应（ＭＵＬＴＩＰＬＥＸ－ＰＣＲ）检测女性生殖道病毒感染的方法具有敏感和特异的优点，用于检测乳头瘤病毒、单纯疱疹病毒和巨细胞病毒，特别适用于常规诊断和普查女性生殖道病毒感染状况。该方法的基本原理是：将三种病毒的特异引物同时加到一个反应体系中，根据病毒ＰＣＲ产物扩增片断的长短来区别是何种病毒感染。本实验的关键问题是解决多种引物在同一反应系统内应不出现交叉反应和非特异扩增，敏感性可这ＦＧ水平，特异性明显优于ＥＬＩＳＡ血清学方法。全部检测过程只需３－４小时，因此，多种引物同时进行ＰＣＲ可以节省至少５０％的试剂消费，同时减轻了病人的负担。

multiplex-PCR method has the superiority of sensitiveness and specificness in detecting papilloma virus,herpes simplex and cytomegalo-virus of female genital tract virus infections.This method is especially suited to clinical routine diagnosis and screening the state of female genital tract viral infection,therefore,privide the laboratory foundations of diagnosis and prevention of these virus infections.The principle is that put these three kind of virus primers into one reaction system at the same time,then determine what kind of infection exists according to the expanding length of PCR products.In this experiment,the key problem is to eliminate cross-reaction and nonspecific expanding,have the sensitivity of fg level,have the superiority to serological methods,eg ELISA 3 to 4 hours are enough for the total testing procedure.The multiplex-PCR method will at least save half of reaction reagent and lighten the burden of patients.