E-钙黏附素基因甲基化与结直肠癌临床病理特征的关系

Relationship between E-cadherin gene methylation and clinicopathological characteristics of colorectal cancer

目的探讨E-钙黏附素(E-cadherin)基因甲基化与结直肠癌临床病理特征的关系。方法采用甲基化特异性巢式PCR技术(nMSP法)研究100例结直肠癌组织及100例癌旁组织E-cadherin基因甲基化与临床病理特征的关系。结果100例结直肠癌组织中有33例呈E-cad基因甲基化阳性(阳性率33.0%),相应的癌旁组织无甲基化。Dukes分期中C、D期组织E-cad基因甲基化阳性率(72.7%)明显高于A、B期(27.3%)(P<0.05);伴肝转移的结直肠癌组织甲基化阳性率(81.8%)明显高于无肝转移者(18.2%)(P<0.01);在肿瘤细胞分化程度、大体类型、年龄、性别和肿瘤部位等其他病理特征,E-cad基因甲基化阳性组与阴性组差异无统计学意义(P>0.05)。结论结直肠癌组织E-cad基因甲基化与淋巴结转移、肝转移和Dukes分期明显相关,E-cad基因甲基化可能是结直肠癌侵袭性增强的原因之一。

Objective To investigate the relationship between methylation in the promoter of the E-cadherin gene and clinicopathological characteristics of the colorectal cancer. Methods Nest methylation-specific PCR （MSP-UMP） was used to detect E-cadherin methylation of 100 pieces of colorectal cancer specimens, and analyze its relation with clinicopathological characteristics. Results In 100 sporadic colorectal cancer specimens, 33 % had detectable E-cadherin methylation, but no detectable methylation was found in all normal tissues. The positive rate of E-cadherin metbylation in the patients with Dukes C and D was significantly higher than that in those with Dukes A and B （P〈0.05）. The positive rate of E-cadherin methylation in the patients with liver metastasis was obviously higher than in those without liver metastasis （P 〈 0.01 ）. There was no statistically significant difference in orther pathological characteristics between E-cadberin methylation positive group and negative group （P 〉 0.05）. Conclusion Methylation in the promoter of the E-cadberin gene was obviously related with lymph node, liver metastasis and Dukes stage. Metbylation of E-cadherin gene may be one of the reasons of the increased invasion of the coloreetal cancer.