摘要
目的探讨腺病毒携带肝细胞生长因子基因(Ad-HGF)不同途径转染对大鼠缺血心肌的影响。方法将75只大鼠随机分为心肌内注射Ad-HGF组、注射携带绿色荧光蛋白(Ad-GFP)组、注射生理盐水空白对照组,心室腔注射Ad-HGF组、注射Ad-GFP组,各组分别于第3、7、142、1、28天各处死3只检查腺病毒的分布情况及进行血管计数。结果心肌内和心室腔注射Ad-HGF组第14、21、28天可见新生血管生成数量显著增加,与对照组相比差异有统计学意义(P<0.05)。心肌内注射Ad-GFP组术后3 d心脏绿色荧光最强,14 d消失,心腔内注射Ad-GFP组术后3 d心脏可见到范围较小的微弱绿色荧光,7 d消失。结论Ad-HGF可明显促进大鼠缺血心肌微小血管生成,心肌内直接注射途径仍优于心腔内给药。
Objective To investigate the effects of adenovirus-mediated hepatocyte growth factor (HGF) on acute ischemic myocardium in different pathways. Methods Seven-five rats were divided into 5 groups randomly. A volume of 100μl normal saline containing 1×10^9 plaque forming units (pfu) of recombinant Ad-HGF, adenovirus-mediated green fluorescence protein (Ad-GFP) or pure saline was injected directly into the ischemic myocardium, respectively. Ad-HGF and Ad-GFP was infused into left ventricular cavity, respectively. The transfected Ad-HGF expression was detected on the day 3, 7, 14, 21, 28 after operation. Results The fluorescent expression in Ad-GFP-injected group was stronger than that in Ad-GFP-infused group. The amount of capillary vessels was counted on the day 3,7, 14, 21,28 after operation. The number of vessels was more significantly increased in Ad-HGF-transfected group than that in control group. Conclusion The administration of Ad-HGF increased the number of vessels in regional myocardium. The transfection rate was lower in Ad-HGF infusion into left ventricular cavity group. The pathway of direct Ad-HGF injection was more effective on acute ischemic myocardium than that of Ad- HGF infusion into left ventricular cavity.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2005年第11期1372-1373,共2页
Chinese Journal of Experimental Surgery
基金
广东省高等学校第二批"千百十工程培养人才"资助项目(2003008)