聚丙烯延胡索酸酯/β-磷酸三钙合成和对骨髓基质细胞生物学行为的影响

Synthesis of poly(propylene fumarate)/β-TCP and culture with bone marrow stromal cells

目的制备聚丙烯延胡索酸酯/β-磷酸三钙(PPF/β-TCP)复合材料.在体外检测PPF/β-TCP材料对骨髓基质细胞(BMSC)的黏附、增殖、成骨能力的影响,对PPF/β-TCP生物材料的性能进行评价.方法二步反应法合成PPF单体,加入β-TCP后进行交联.骨髓基质细胞在PPF/β-TCP材料上培养2、4、6、8、10、12 h后胰蛋白酶消化,检测黏附细胞数.BMSC和PPF/β-TCP材料复合培养,用MTT法检测BMSC的增殖,绘制生长曲线,检测碱性磷酸酶的含量.结果BMSC在PPF/β-TCP材料上黏附,2～8 h细胞数增加,到10 h黏附数达最高,约为68%.BMSC在PPF/β-TCP材料上生长,第4、5天为对数生长期,第7、8天进入平台期.BMSC种植在PPF/β-TCP后上表达ALP并不断增加.结论 PPF/β-TCP生物降解材料对BMSC的黏附、增殖、成骨功能无不良影响,是一种有前途的生物降解材料.

Objective To prepare the biomaterial poly（ propylene fumarate）/β-TCP （PPF/β-TCP） and in vitro test its effect on adhesion, proliferation and osteogenic potential of bone marrow stroreal cells （BMSC） so as to evaluate the characteristics of PPF/β-TCP. Methods PPF was synthesized and crosslinked after adding β-TCP. BMSC were cultured on PPF/β-TCP and collected within 12 h every two h to calculate the adhesion number. The proliferation of BMSC on PPF/β-TCP was tested by MTT method, growth curve was drawn, and the activity of alkaline phophatase was assayed. Results BMSC could adhere to PPF/β-TCP, the number of cells increased within 10 h and reached to 68% at 10 h. BM-SC could proliferate on PPF/β-TCP, entered the log phase on the day 4 and day 5, reached plateau phase on the day 7 and day 8. BMSC could express ALP on PPF/β-TCP. Conclusion PPF/β-TCP has no adverse effect on BMSC and is a promising biomaterial.