高强度窄谱蓝光对寻常痤疮表皮分离菌的抗菌作用研究

In vitro Effect of High Intensity Blue Light on Bacteria Isolated from Patients with Acne Vulgaris

目的评价高强度窄谱蓝光对寻常痤疮致病菌的抗菌作用。方法取67例轻、中度痤疮患者皮损内容物进行细菌培养、分离并鉴定菌种。用高强度窄谱蓝光对培养所得的各种细菌进行照射,观察照光前后细菌数量、形态及超微结构的变化。结果皮损处检出痤疮丙酸杆菌(32．4％)、颗粒丙酸杆菌 (6．9％)、表皮葡萄球菌(35．3％)、金黄色葡萄球菌(4．9%)、溶血葡萄球菌(7．8％)、孔氏葡萄球菌(1．0％)、棒状杆菌(9．8％)及肺炎克雷伯菌(1．0％)和B群链球菌(1．0％)。照光后痤疮丙酸杆菌和表皮葡萄球菌较未照光组菌量显著减少(P<0．05),菌落明显变小。结论高强度窄谱蓝光有抗痤疮表皮分离菌作用。

Objective To assess the in vitro effect of high intensity narrow-band blue light on bacteria isolated from patients with acne vulgaris （AV）. Methods Bacteria were isolated by culture from the aene lesions of 67 patients with mild to moderate AV. Selected bacterial strains were chosen for irradiation with the blue light. The morphology and number of colony formation units （CFUs） as well as the uhrastructure of the bacteria were observed before and after the irradiation. Results Propionibacterium acnes （P. acnes） （32.4%）, P. granulosum （6.9%）, Staphylococcus epidermidis （S. epidermidis ） （35.3%）, S. aureus （4.9%）, S. heamolyticus （7.8%）, S. cohnii （1.0%）, Corynebacterium spp. （9.8%）, Klebsiella pneumoniae （1.0%） and B group Streptococci （1.0%） were isolated from the lesions. In vitro investigation revealed that the irradiation significantly reduced the number of CFUs of P. ache and S. epidermidis. Conclusion High intensity narrow-band blue light is effective against bacteria isolated from AV lesions.