外源性IFN-β基因对裸鼠SHG44胶质瘤的诱导凋亡研究

The Study of Eukaryotic Vector Encoding IFN-βGene Inducing Nude Mice of Human SHG44 Glioma Apoptosis

目的:探讨β干扰素基因对人脑胶质瘤的诱导凋亡作用,以及人β干扰素裸DNA治疗人脑胶质瘤的可行性和作用机制。方法:建立裸鼠SHG44胶质瘤模型,用脂质体包埋法将IFN-β基因的真核表达载体pSV2IFNβ注入裸鼠皮下SHG44脑胶质瘤,观察肿瘤生长情况并计算肿瘤体积,通过免疫组化、TUNEL染色以及电镜,了解IFN-β基因诱导人脑胶质瘤细胞凋亡的作用。结果:IFN-β在荷瘤裸鼠瘤体内获得表达,裸鼠皮下胶质瘤生长受到抑制,并诱导SHG44胶质瘤细胞凋亡。结论:IFN-β裸DNA能够抑制人脑胶质瘤生长并诱导胶质瘤细胞凋亡,该实验为IFN-β基因治疗人脑胶质瘤的应用奠定了初步基础。

Objective： To elucidate the effects of interferonβ （IFN-β） gene inducing human gliomas apoptosis and to discuss the feasibility and effect mechanism of interferonβnude DNA treatment for human gliomas. Methods： The SHG44 glioma model of nude mice was establishecd. Eukaryonic expression vector （pSV2IFNβ） for IFN-β packaged by liposome was injected into SHG44 glioma of nude mice. Observe the glioma growth and calculate tumor size. The effects of IFN-β gene inducing human gliomas apoptosis was realized by immunohistochemistry, tunel and electron microscope. Restilts： The expression of IFN-β gene in the glioma nude mice was determined. The glioma growth of nude mice was inhibited. IFN-β can induce glioma cell SHG44 apoptosis. Conclusion： IFN-β gene can inhibit the growth of human gliomas and induce the apoptosis of human glioma cells. The experiment provides an elementary basis for the treatment of glioma by using IFN-βgene.