Rb、p16、CyclinD1基因蛋白在大肠癌中的表达及临床意义

The Expression of Rb, P^(16) and CyclinD_1 proteins in Large Intestine Carcinoma and Its Clinical Significance

目的:探讨抑癌基因Rb、p16和癌基因CyclinD1在大肠癌中的表达及临床意义。方法:采用流式细胞分析技术(Flowcytometry,FCM),定量研究在大肠癌与正常粘膜中Rb、p16、CyclinD1的表达情况,所有数据换算成荧光指数(FluorescenceIndex,FI)值后,利用SAS软件包进行统计分析。结果:1)30例大肠癌组织与正常粘膜中Rb、p16、CyclinD1FI(x±s)值经t检验,均P<0.01;2)以Rb表达阳性、阴性进行分组,在Rb阳性下,p16与组织学分级呈负相关,CyclinD1与组织学分级呈正相关,p16与淋巴转移呈负相关,均P<0.01。在Rb阴性下,Rb与淋巴转移呈负相关,p16与淋巴转移呈正相关,而CyclinD1与淋巴转移呈负相关,均P<0.01。结论:1)Rb、p16、CyclinD1在大肠癌、正常粘膜组织中的表达存在显著差异;2)在Rb阳性下,p16失活、缺失或突变会导致组织的恶性程度增加且易发生淋巴转移,CyclinD1的激活会导致组织的恶性程度增加;3)在Rb阴性下,各种结果显示:负反馈机制在其中可能起作用。

Objective： To discuss the expression of Rb, p16 and CyclinD1 proteins in large intestine carcinoma and its clinical significance. Methods： The expression of Rb, P16 and CyclinD1 in the colon-rectal malignant carcinoma and normal mucosa were measured by flow cytometry （FCM） and all the data were analysed by SAS after the data were transformed to FI （fluorescence index）. Results： a） FI （^-x±s） of Rb,P16 and CyclinD1 in the colon-rectal malignant carcinoma with FI （^-x±s） of normal mucosa are analyzed by T test （P〈0.01）. b） The correlations between Rb, P16 and CyclinD1 and the degree of malignancy were further researched after the expression of Rb was classified, depending on whether the expression of Rb was positive or negative. When Rb was the positive expression, namely, Rb FI〉1.0, the expression of P16 will have a negative correlation with the grade of histology. CyclinD1 had a positive correlation with grade of histology. And less expression of P16 has a significant correlation with the lymphatic metastasis as Rb FI〉1.0 and above, P〈0.01. c） When the expression of Rb was negative, Rb had a negative correlation with lymphatic metastasis, P16 had a positive correlation with lymphatic metastasis and CyclinD1 had a negative correlation with lymphatic metastasis, P〈0.01. Conclusions： a） There are a significant difference in the expressions of Rb, P16 and CyclinD1 between colon-rectal carcinoma group and normal mucosa group, b） When the expression of Rb is positive, the inactivation, lost, or mutation of P16 will lead to more and more malignant and easily cause lymphatic metastasis. The degree of activeated CyclinD1 will lead to more and more malignancy, c） When the expression of Rb is negative, all the results indicate that negative feedback maybe affect the expressions of Rb, P16 and CyclinD1. But, owing to the only samples in 4 cases of this group, the results need more studies by enlarging the samples.