survivin在非小细胞肺癌中的表达

Expression of survivin and its clinical significance in non-small cell lung cancer

目的:探讨survivin蛋白在非小细胞肺癌(nonsmallcelllungcancer,NSCLC)中的表达及其与临床病理特征和突变型p53表达的关系。方法:分别利用鼠抗人survivin单克隆抗体和p53单克隆抗体采用ENVISION免疫组织化学方法检测survivin,P53蛋白在61例NSCLC中的表达。结果:survivin蛋白在NSCLC中的阳性染色位于细胞浆和/或细胞核,总阳性率为77.0%(47/61),其中42.6%(26/61)为胞浆胞核双阳性,26.2%(16/61)为仅胞浆阳性,8.2%(5/61)为仅胞核阳性。survivin蛋白在肺鳞状细胞癌组织中胞浆胞核双阳性率为57.6%(19/33),显著高于其在肺腺癌中的表达25.0%(7/28),二者比较,差异有统计学意义(P<0.05)。P53蛋白表达阳性组与阴性组中,survivin蛋白的胞浆胞核双阳性率分别为54.3%(19/35)和26.9%(7/26),二者比较,差异有统计学意义(P<0.05)。survivin在NSCLC中的表达与患者性别、年龄、临床分期、组织分化程度、有无淋巴结转移及预后等临床病理特征间无明显关系。结论:survivin在NSCLC中高表达,与病理类型相关,主要表现在鳞状细胞癌中的表达高于腺癌,可能通过抑制肿瘤细胞凋亡并促进肿瘤血管形成,在NSCLC的发生发展中起重要作用。同时抑癌基因p53失活与survivin的表达可能在NSCLC癌变中起协同作用。

Objective： To investigate the expression of survivin in non-small cell lung cancer （NSCLC） and analyze its relationship with clinic opathological features and the expression of p53. Methods ： The expression of survivin and p53 in 61 cases of NSCLC were detected by immunohistochemistry using monoclonal antibodies against human survivin protein and P53, respectively. Results： The expression of survivin in NSCLC could be detected in cytoplasm and/or in nucleus. The overall immunoreactivity was found in 77.0% （47/61） of tumors. Cytoplasmic, nuclear and both immunoreactivities were present in 26.2% （ 16/61 ） , 8.2% （5/61） and 42.62% （26/61） respectively. The cytoplasmic and nuclear immunoreactivities in squamous cell carcinoma （57.6% , 19/33） were higher than that in adenocarcinoma （25%, 7/28） and there was significant difference （P 〈0.05）. The positive expression rates of survivin in P53 positive and negative were 54.3% （ 19/35 ） and 26.9% （ 7/26 ） respectively and there was significant difference （P 〈 0.05）. Survivin expression levels did not correlate with the patient＇s gender, age, clinical stage, histological differentiation, lymph node metastasis status or survival. Conclusion： survivin was overexpressed in NSCLC. It correlated with pathological type that mainly showed the positive expression rate in squamous cell carcinoma was higher than that in adenocarcinoma. It may play an important role in the carcinogenesis and development of NSCLC by inhibiting tumor cell apoptosis and facilitating angiogenesis. Inactivation of antioncogene p53 and expression of survivin may play synergetic roles in carcinogenesis of NSCLC.