摘要
目的:探讨质子磁共振波谱(1H-MRS)在帕金森病(PD)大鼠模型纹状体内的变化及在PD大鼠模型中的应用。方法:选用雄性Wistar大鼠12只,向其右侧纹状体内两点注射6-羟基多巴(6-OHDA)制作PD大鼠模型,5周后颈部皮下注射阿朴吗啡诱导其向健侧旋转,每分钟大于7圈者为成功模型,获6只。对照组6只大鼠以同样方法注射等剂量的生理盐水。利用BIOSPEC47/30核磁共振仪(4.7T)对双侧纹状体行1H-磁共振波谱检测,检测后立即处死动物并行黑质及纹状体酪氨酸羟化酶(TH)免疫组织化学染色。结果:模型组大鼠右侧黑质致密部TH阳性神经元及纹状体TH阳性纤维明显减少(P<0.01),证明模型制作成功。模型组大鼠右侧(毁损侧)纹状体内NAA/Cr比值明显低于左侧及对照组的两侧(P<0.05),对照组两侧NAA/Cr比值无显著差别。两组大鼠双侧纹状体内Cho/Cr比值均无显著差别。结论:1H-MRS可以作为一种无创性的新技术,为偏侧大鼠PD模型纹状体部位的神经细胞的病理学改变提供有价值的信息。
Objective： To explore the applied value of ^1H-magnetic resonance spectroscopy （^1H-MRS）in rat model of Parkinson^s disease （PD）. Methods： 12 Wistar rats were divided into model and control groups. On the 5th week, after 6-hydroxydopamine injected into right striatum, ^1H-MRS on the striatum was taken and then tyrosine hydroxylase（TH） immunostatining was used to observe the changes of the neurons and neuritis in substantia nigra and striatum. Results： TH positive neurons and neurites in the right substantia nigra compacta （SNc） and striatum of the rat model of PD were decreased （P〈0.01）, which proved successful PD models. The NAA/Cr ratio in the right striatum of model group was lower than that of the left side and the both sides of control group（P〈0.05）. While there was no difference between the two sides in the control group （P〉0.05）. The radio of Cho/Cr showed no difference between the two groups （P〉0.05）. Conclusion： ^1H-MRS is a noninvasive technique that can provide useful information concerning the pathological changes of nerve cells in the striatum of PD rat models. It is a helpful method for the PD study in rat model.
出处
《山东大学学报（医学版）》
CAS
北大核心
2005年第9期769-772,共4页
Journal of Shandong University:Health Sciences
基金
国家教育部高等学校博士学科点专项科研基金资助项目(20040422040)
