乙酰肝素酶反义寡核苷酸对SGC-7901细胞MMP-2和TIMP-2表达的影响 Ⅰ.乙酰肝素酶反义寡核苷酸对SGC-7901细胞MMP-2和TMP-2表达的影响

Influence of MMP-2 and TIMP-2 expressions in SGC-7901 by heparanase antisense oligodeoxynucleotide

目的:探讨乙酰肝素酶(HPA)反义寡脱氧核苷酸(AS-OND)对人胃癌细胞株SGC-7901细胞基质金属蛋白酶-2(MMP-2)及组织金属蛋白酶抑制剂-2(TIMP-2)表达的影响。方法:采用脂质体介导法将HPAAS-ODN转染SGC-7901细胞G用RT-PCR检测HPA-mRNA表达,用免疫细胞化学的方法检测细胞内MMP-2和TIMP-2的变化。结果:AS-ODN转染48h后GSGC-7901细胞内HPA-mRNA表达下降(P<0.01),MMP-2的表达量亦减少(P<0.01)。结论:HPAAS-ODN可以有效地抑制胃癌细胞MMP-2的表达。

Objective： To evaluate the effect of heparanase antisense oligodeoxynucleotide （ASONI）） on the expression of CD44v6 in human gastric carcinoma cell line SGC-7901. Methods： Human gastric aden,carcinoma cell line SGC-7901 was transfeeted with liposome-mediated heparanase AS-OND, The expression of heparanase mRNA was detected after transfection by semi-quantitative total RNAs RT-PCR. The change of CD44v6 was ohserved by using immunocytochemistry. Results： The heparanase mRNA expression and CD44v6 expression of SGC-7901 cells after treated with AS-ODN 48 hours at different final concentrations were significantly decreased compared with those in the controls （P〈0.01）. The expression rate of CD44v6 was 69.4% before transfection, and 56.5%, 54.1%, 46.5% and 41.3% after treated with 0.1 μmol/ L, 0.2 μmol/ L. 0.4 μmol/ L and 0.8 μmol/ L AS-ODN of the final concentrations, respectively, Conclusion： Heparanase AS-ODN has a significant inhibitory, effect on CD44v6 expression in human gastric carcinoma cell line SGC-7901 in a dose-dependent manner.