DNA分子标记在猕猴桃上的应用

Advances in research on application of DNA molecular markers in Actinidia

猕猴桃为中国半特有属植物,由于雌雄异株,自然杂交明显,染色体倍性复杂等原因,用常规方法对其进行种类(品种)鉴定、系谱分析、遗传多样性研究等难度较大。DNA分子标记具有许多优点,它的发展和应用为猕猴桃研究提供了一条有效的途径。就DNA分子标记在猕猴桃系谱分析与品种(品系)鉴定、性别鉴定、染色体起源分析、胞质遗传与系统发育研究、遗传多样性研究和分子遗传图谱构建等方面的应用进行了详细阐述,同时指出了DNA分子标记在猕猴桃研究中存在的问题和今后研究工作的重点。

Actinidia is a half-endemic genera in China. It is difficult to be studied completely by traditional methods, due to its sexual variation and chromosome, morphological characteristics and genetic diversity. DNA molecular markers have many advantages compared with the traditional methods, so they are efficient techniques in Actinidia research. This paper summarizes the progress in research of Actinidia using DNA molecular markers, including pedigree analysis, cultivar and strain identification, gender identification, chromosome ploidy and derivation analysis, cytoplasmic heredity and phylogene research, genetic diversity study and construction of genetic linkage map. The current problems and prospects of the application are also discussed.