生物柴油的酶促合成研究

Enzymatic synthesis of biodiesel

对固定化脂肪酶催化大豆油甲酯化反应生产生物柴油的工艺进行了研究。考察了反应条件如油醇摩尔比、甲醇的流加、温度、有机溶媒用量以及底物预处理对生物柴油产率的影响，并对产物进行了分析。结果表明，当采用乳化处理后的大豆油为底物，正已烷作为有机溶媒，固定化脂肪酶用量为原料油质量的10%，反应温度为40℃，每隔5h流加甲醇1次，每次按油醇摩尔比1：1流加，共流加3次时，反应的转化率最高可达到94%。

Immobilized lipase was used to catalyze transesterification reaction to produce biodiesel. The effects of methanol toxicity, temperature, solvent amount and pretreatment of substrate were studied and the component of product was analyzed. The results show that the transesterifieafion degree under the optimal parameters and process can reach 94%.