壳聚糖对口蹄疫DNA疫苗黏膜免疫的影响

Effects of chitosan on the FMDV-DNA mucosal immunization

以寻求有效DNA疫苗黏膜免疫途径为目的,利用带有负电荷的具有黏膜粘附性多糖-壳聚糖来包裹口蹄疫DNA疫苗pcD-VP1形成纳米级颗粒,用此颗粒经滴鼻、口服、直肠和生殖道4种黏膜免疫小鼠,检测小鼠的针对口蹄疫的体液和细胞免疫水平。结果显示:壳聚糖/pcD-VP1滴鼻免疫小鼠,抗体的高峰来临比只免疫pcD-VP1提前2周,直肠免疫处理中也呈现如此效果;经口服和生殖道途径的免疫,均没有呈现比pcD-VP1免疫组更好的抗体水平。但壳聚糖/pcD-VP1在生殖道和直肠免疫途径里均比pcD-VP1免疫组P.P.结的T细胞增殖水平高。所以,除口服途径以外,在滴鼻、直肠和生殖道免疫中,壳聚糖对质粒的包裹均一定程度上提高了DNA疫苗黏膜免疫的效果。

The aim of our investigation was to improve the effectiveness of DNA vaccines against foot-mouth disease virus （FMDV） infection on mucosal immnunization. We use chitosan-a cational polymer as mucosal immunization agent. To see if chitosan-DNA vaccine could effectively prevent development of foot-mouth disease virus （FMDV）, FMDV DNA vaccine pcD-VPlwere used at this research. Chitosan-pcD-VP1 （chi-pcD-VP1）vaccine were prepared by complexing chitosan with pcD-VP1 DNA and administrated to Kunmingbai mice nasally, orally, rectally and vaginally with 150 μg DNA at 14 days intervals, 50 μg DNA each time one Mice. The result shows that vaginal IgA of intranasal and intralrectum immunized chitosan-DNA mice were advance higest level about 2 weeks. P.P. T cell proliferation shows significant enhanced at rectum and vaginal vaccined chitosan-DNA groups.