肝硬化患者肝组织中增殖细胞核抗原的检测

Detection of PCNA in liver tissues of cirrhosis

应用免疫组化法对３２例肝硬化活检或尸检肝组织中的增殖细胞核抗原（ＰＣＮＡ）进行了检测。２３例缺如（７１．９％），９例阳性（２８．１％），阳性细胞数少且与各例相差较大，多位于假小叶的边缘。相比之下，亚急性重型肝炎和重型慢性活动性肝炎肝组织中有较多的ＰＣＮＡ阳性细胞（Ｐ＜０．０５）。结果表明，肝硬化时肝细胞的增殖状态是相当低下的。

Abstract The speimens of Liver biopsy and autopsy of 32 cases of active cirrhosis (AC) were detected with imMunohistochmically stained for proliferating cell nuclear antigen (PCNA)to evaluate the proliferative activity of hepatocytes.The proliferative activity was completely negative in the most of specimens (23/32,71.9%),hepatocytes positive for PCNA/cyclin in the rest 9 cases(9/32,28.1%)were found mostly in the area nest to the fibrotic septum and varied greatly in the different pseudolobules. As controls ,we also have detected some specimens of the patients with viral hepatitis B,including 16 cases of subacute fulminat heptitis (SFH),14 cases of chronic active hepatitis in the heavy type (CAHH),and 11 cases of chronic fulminant hepatitis (CFH),and found that PCNA positivity rate in the SFH and CAHH was higher that of the patients with in AC and CFH(P<0.05).These results show that the proliferative activity was very lower in patients with the AC.