摘要
免疫球蛋白重链（ＩｇＨ）重排，可作为Ｂ淋巴细胞白血病的基因标志，为进一步了解急性非淋巴细胞白血病（急非淋）ＩｇＨ重排情况，用聚合酶链反应（ＰＣＲ）联合地高辛标记ＪＨ探针Ｓｏｕｔｈｅｒｎ杂交，检测４１例急非淋病人ＩｇＨＣＤＲ－Ⅲ区域。７例（１７．１％）ＰＣＲ扩增阳性，此７例阳性病例均经Ｓｏｕｔｈｅｒｎ杂交证实，本实验的灵敏度为１０－４～１０－５水平。１２例完全缓解病例中３例（２５．０％）重排阳性，此３例均于半年内临床复发。结果表明，ＩｇＨ重排并不局限于Ｂ淋巴系白血病，也可发生于急非淋病人，其机制可能是，部分急非淋起源于较原始的多向造血祖细胞或除髓系白血病克隆外。
Immunoglobulinheavychain(IgH)generearangementservesasamarkerofclonalityinBlymphoproliferativemalignancies.InordertostudytheIgHrearangedgeneinacutenonlym-phoblasticleukemia(ANLL)patientswecombinepolymerasechainreaction(PCR)withSouthernblottodetect41ANLLpatientsand7ofthem(17.1%)werefoundtohaveIgHrearrangementbyPCRam-plification.Althese7positivecaseswereconfirmedbySouthernblot.Thesensitivityofthismethodwas10-4～10-5level.In12patientswithcompleteremission,3(25.0%)werefoundtohaveIgHrear-rangedgene.Althese3caseshadclinicalrelapsewithin6months.OurresultsshowthatIgHrear-rangementnotonlymayoccurinlymphoblasticleukemiaofBlineage,butalsocanbefoundinANLL.ThemechanismmaybethatinsomeANLLpatients,theleukemictransformingeventmightinvolvestemcelscapableofbothBcelandmyeloiddiferentiationorANLLmightdiferentiatealongdiferentlineagewithpredominantappearanceofoneortheothersubcloneinthecourseofthedisease.
出处
《中华内科杂志》
CSCD
北大核心
1996年第9期587-590,共4页
Chinese Journal of Internal Medicine
基金
军队八五医药卫生科研基金
关键词
聚合酶链反应
白血病
免疫球蛋白类
PolymerasechainreactionLeukemiaImmunoglobulinsheavy-chain
