东北红豆杉紫杉醇的提取纯化与HPLC检测

Improved Purification Determination of Taxol

对东北红豆杉中紫杉醇的提纯与检测技术进行了研究,确立了固相萃取、液-液萃取、正己烷沉淀、硅胶柱层析分离、4℃、V(甲醇)∶V(水)=3∶1相中冷却放置48 h的紫杉醇提取纯化技术,经高效液相色谱法检测,产品纯度达93%以上.

The process of extracting taxol from the northeastern Taxus leaves was analyzed and discussed through the experiments. The methods of extraction and elimination impurity in taxol pretreatment by solid phase extraction, liquid-liquid extraction, silical-gel chromatography precipitation, the methanol/water（3：l） of 4 ℃ crystallize mutually to cool off to place a product for, making taxol crystallized Withdraw to turn the technique purely. Making use of the thin layer launch the method with efficiently liquid mutually the H.PLC method proceeds the examination can get 93% above pure degree in the system.