拟南芥MT-II过量表达提高抗旱性(英文)

Expression of Metallothionein-II in Arabidopsis thaliana improve desiccation tolerance

富含巯基的植物II型金属硫蛋白(MT)对植物抵抗重金属胁迫具有重要作用,其中一个可能机制是金属硫蛋白可能猝灭重金属引起的氧化胁迫。利用转MT-II基因和野生型拟南芥(Arabidopsis thaliana)植株来对比研究MT在胁迫过程中通过清除氧自由基,特别是H2O2而对植物抗旱性的影响。研究表明,转基因型拟南芥能有效维持体内氧化—还原势,减少MDA的产生,从而缓解干旱胁迫引起的伤害,提高抗旱性。

Metallothionein-Ⅱ （MT-Ⅱ） has been identified to play an important role in the exposure of plant to heavy metal like Cd and Cu. One of the mechanisms of MT＇ s proposed to protect plants during this process is that it can quench the reactive oxygen species （ROS） activated by heavy-metal exposure. Here we demonstrated the effect of expression of MT-Ⅱ in Arabidopsis thatiana on oxidative stress and damage extent during drought stress. The RT-PCR results suggested the implication of MT-Ⅱ against drought stress in the transgenic Arabidopsis. Measurements of H2O2 and malondialdehyde （MDA） indicated that over-expression of MT-Ⅱ in A. thaliana improved desiccation tolerance.