烟草叶片愈伤组织BECTLINⅠ基因的表达(英文)

Expression of BECTLIN Ⅰ gene in the leaf callus of Nicotiana benthamiana

分别以烟草盆栽实生苗和组培继代苗的叶片为外植体,在MS(1.0 mg.L-16-BA与0.5 mg.L-12,4-D)培养基中诱导形成的愈伤组织为材料,检测与细胞程序性死亡有关的BECT-LINⅠ基因。RT-PCR检测表明,烟草盆栽实生苗和组培继代苗的叶片及其愈伤组织均有BECT-LINⅠ基因的表达,但组培继代苗叶片愈伤组织的BECTLINⅠ基因表现出持续、稳定的表达,说明培养基中的激素可能对BECTLINⅠ基因的表达产生影响,同时表明烟草叶片愈伤组织形成过程中可能产生了细胞程序化死亡,其结果可能导致烟草叶片原初形态不同、功能各异的细胞在脱分化阶段形成了形态结构和功能完全相似的胚性细胞。

We induced the callus from the leaves of Nicotiana benthamiana cultured in the MS medium and grew in the green house. Then we used the callus to detect the expression of BECTLIN Ⅰ gene which was correlated with Programmed Cell Death （PCD）. The detection of RT-PCR showed that the fragment of BECTLIN Ⅰ gene cDNA was amplified in the leaves of the in-vitro plantlets subcultured in the medium and those of the plotted plant seedling, but BECTLIN Ⅰ gene expressed continually and steadily in the leaves callus of the plantlets. This probably indicated that auxins in the medium had some effect on the expression of BECTLIN Ⅰ gene. At the same time, PCD might occur in the course of the formation of the leaves callus, and in the state of dedifferentiation there probably appeared the embryoblasts with the same configuration and function.