一次接种HA DNA保护小鼠抵抗B型流感病毒攻击

A Single Inoculation of HA DNA Vaccine to Protect Mice from Influenza B Virus Attack at Lethal Dose

为详细探讨小鼠不同次数接种B型流感病毒HA DNA疫苗后免疫应答情况,以及CpG基序的免疫佐剂效果,采用不同剂量HA DNA,1次或2次(间隔3周)电击法免疫BALB/C小鼠。初免4周后(或二免加强免疫1周后)用致死量流感病毒(B/Ibaraki/2/85)攻击。研究发现:①100μg HA DNA一次接种的小鼠全部存活;②经含有CpG基序的DNA免疫的小鼠体内诱导产生的抗HAIgG抗体更高,小鼠体重丢失更少。这些结果说明,1次接种100μg HA DNA疫苗可以使小鼠抵抗致死量B型流感病毒攻击,CpG基序能够增强HA DNA疫苗的免疫保护作用。

In order to investigate the immune response induced by different times of inoculation with influenza B virus HA DNA vaccine and the adjuvant effect of CpC motif, BALB/c mice were immunized by electrical shock once or twice at a three-week interval with plasmid HA DNA at different doses. Four week after the first immunization （or one week after the reinforced immunization）, the mice were attacked with a lethal dose of influenza virus （B/ Ibaraki/2/85）. The experiments showed that 1. mice immunized with a single inoculation of 100μg HA DNA all survived the lethal dose attack; 2. mice immunized with CpC-enxiehed HA-plasmids exhibited a higher amount of HA specific IgG and lower weight loss than those with HA-plasmids without CpC motif. Therefore, a single inoculation of 100μg HA DNA could provide complete protection from the lethal dose of influenza B virus attaekA DNAHA and the CpC motif could augment the immune response induced by HA DNA vaccine.