南瓜种质资源遗传多样性的RAPD分析

Analysis of Genetic Diversity in Cucurbita by RAPD Markers

应用RAPD技术对70份南瓜种质进行遗传多样性分析,从100条随机引物中筛选出21条引物,共扩增出168条带,其中157条为多态带,比率为93·5%。系统聚类分析将70份南瓜种质分为3大类:中国南瓜、美洲南瓜、印度南瓜,与传统分类学的结果相符。同时在3大类群内又将种质进行细分,第1类分为3组,第2类分为6组,第3类分为5组,其结果与地域来源和形态特征有一定的相关性。主成分分析与系统聚类分析结果基本一致,但系统聚类分析在揭示密切相关的个体间的关系上能提供更丰富的信息。

In this study, the genetic diversity among 70 accessions in cucurbita was analyzed by using RAPD marker and the genetic relationship among the accessions was revealed by cluster analysis and principal coordinate analysis respectively. 21 polymorphie primers, screened out of 100 random primers, were used and produced 168 reproducible bands. Of them, 157 bands （93.5%） were polymorphie. The cluster analysis divided the germplasm used in this study into 3 groups： Cururbita moschata, C. pepo, and C. maxima, which was in agreement with the traditional taxonomy on Cucurbita. Moreover, Cururbita rnoschata was divided further into 3 subgroups, C. pepo into 6 subgroups, and C. maxima, into 5 subgroups respectively by cluster analysis. The clusters were greatly associated with the origins and the morphologic characters of the tested germplasm. The results obtained by the principal coordinate analysis were consistent with those of cluster analysis. However, cluster analysis could supply more abundant informations than principal coordinate analysis on revealing the relationship among the closely related accessions.