槲皮素对甘蓝自交不亲和性及SRK活性的影响

Effects of Quercetin on Self-incompatibility and SRK Activity in Brassica oleracea L.

对甘蓝自交不亲和系‘02-E1’,‘02-A1’,‘02-59’开花前两周的花蕾进行不同浓度的槲皮素处理,开花后自交。与花期对照相比,3个自交不亲和系的亲和指数差异达到显著性水平,其中以1500μmol·L-1槲皮素溶液处理效果最佳,在02-E1系中其亲和指数由原来的0·03增至4·35,完全转化为了自交亲和系。采集开花前1d的花蕾切取柱头进行SRK活性测定,结果表明,槲皮素处理后可显著抑制甘蓝自交不亲和系柱头中SRK活性,从而阻止了自交不亲和性的信号传导,达到了克服甘蓝自交不亲和性的目的。

In this experiment, the Self-incompatible （SI） lines buds were treated with different concentrations of quercetin solution （500, 1 000 and 1 500μmol· L^- 1 ）. The results showed quercetin could overcome the SI of Brassica oleracea L. significantly. What＇s more, the most seeds per pollinated flower were obtained with the treatment of 1 500 μmol· L^-1 quercetin solution among three concentrations. In addition, the self-compatible （SC） index of 02-E1 SI line increased from 0. 03 to 4. 35, which similarly to bud pollination. Quercetin acted as a kind of Sreceptor kinase （SRK） inhibitor, it can prevent the signal conduction of SI since it reduced SRK activity by spraying certain concentration quercetin. So it is feasible to overcome the SI by spraying quercetin.