测定百合花粉生命力的液体培养基研究

Studies on the Culture Solution for Lily Pollen Vitality Test

以东方百合品种'索蚌'的新鲜花粉为试材,利用单因子试验比较了液体培养基中蔗糖浓度及硼、钙、镁、钾对其萌发的影响.在此基础上进行正交试验,比较蔗糖、H3BO3、CaCl2对百合花粉萌发的影响.试验结果表明:蔗糖和H3BO3对百合花粉萌发有极显著影响.适宜的花粉培养液为:蔗糖100 g·L-1 +H3BO3 20 mg·L-1+CaCl2 20～30 mg·L-1.纯水培养液会造成花粉原生质体破裂,内含物外流;高浓度的蔗糖和盐会造成原生质体失水萎缩,质壁分离,这两种情况都抑制花粉萌发.

The fresh pollen of oriental hybrids ＇Sorbonne＇ was employed as experimental material to carry on the studies on culture solution for the determination of lily pollen viability. Comparisons were made among the effects of different concentration of sucrose, boron, calcium, magnesium and potassium on the germination of lily pollen through simple factorial experiment. And an orthogonal design was used on that basis to compare the effects of sucrose, boron and calcium on the germination of lily pollen. Results showed that sucrose and boron have great effects on the germination of lily pollen. The optimum culture solution was sucrose 100 g · L^-1 ＋H3BO3 20 mg· L^-1 ＋CaC12 20 -30 mg· L^-1. When cultured in pure water, the protoplasm of the pollen would split, and the substance inside would flow out; when cultured in the solution with high concentration of sucrose or salt, the protoplasm would dehydrate and separate from the cell wall. These two would inhibit the germination of lily pollen.