中药祛癍方醇提物对大鼠组织和红细胞的抗氧化活性

The activities of ECMH on antioxidation in tissues and erythrocytes of rat

目的:研究中药祛癍方醇提物(ECMH)的抗氧化活性。方法:用.OH生成系统Fe2++抗坏血酸诱导大鼠心、肝、肾组织匀浆脂质过氧化,用硫代巴比妥酸(TBA)比色法测定丙二醛(MDA)含量;用氯化硝基四氮唑蓝(NBT)还原法测酵母多糖A刺激大鼠中性白细胞产生的O2.;用分光光度法测H2O2诱发的大鼠红细胞溶血度。结果:中药祛癍方醇提物抑制MDA及O2.生成;对抗溶血反应。结论:中药祛癍方醇提物通过清除.OH,O2.及H2O2发挥抗氧化活性。

OBJECTIVE To study the activities of the alcohol extractive from a dose of Chinese medical herbs for eliminating senile plaque （ECMH） on antilipoperoxidation. METHODS The peroxidation in homogenates of heart, liver and kidney of rat was induced by OH generation system Fe^2＋ ascorbic acid,malondialdehyde（MDA）was determined using TBA colorimetric method. Superoxide （O2^- ）from zymosan-stimulated neutrophils of rat was measured by NBT reduction method. H2O2^- caused hemolysis of erythrocytes was measured spectrometrically. RESULTS ECMH inhibited MDA and O2^- generation and antagonized hemolysis. CONCLUSION ECMH exert their antioxidation by scavenging · OH,O2^- and H2O2^- in biological system.