大肠癌VEGFR-3与Prox1的相关性研究

The relationship between the expression of VEGFR-3 and Prox1 in colorectal cancer

目的探讨VEGFR-3和Prox1在大肠癌组织、正常黏膜组织和淋巴结中的表达及其相互关系。方法采用实时定量RT-PCR检测63份直肠癌远端肠壁组织,156枚系膜淋巴结,配对肿瘤组织和邻近正常黏膜组织各13份的VEGFR-3,Prox1的表达。结果VEGFR-3和Prox1的mRNA含量中位数在肿瘤远端肠壁组织分别为22.08和544.97,两者有明显相关性(r=0.716,P=0.000 1)。在淋巴结分别为33.81和439.16,两者有明显相关性(r=0.618,P=0.000 1)。在肿瘤组织分别为0.65和5877,两者没有相关性(r=-0.280,P=0.353)。结论VEGFR-3与Prox1在正常肠壁组织和系膜淋巴结的新生淋巴管形成中可能有协同作用,但在肿瘤组织不存在协同关系。

Objective To investigate the expression and relationship between VEGFR-3 and Proxl in the tissues of tumor, normal mucosa and lymph nodes from the surgieal resection specimens of colorectal cancer. Methods Sixty- three normal intestinal tissues distal to rectal tumor, 156 mesentery lymph nodes and 13 matched pairs of tumor and adjacent nontumorous mucesa samples from patients with colorectal cancer were measured for VEGFR- 3 and Proxl expression using quantitative realtime RT-PCR. Results The median of VEGFR- 3 and Proxl mBNA content in intestinal tissues distal to rectal tumor was 22.08 and 544.97 respeetivdy, between which a significant correlation was found （r =0.716, P=0.000 1）. It was 33.81 and 439.16 in mesentery lymph nodes respeetivdy, and they were significantly correlated to each other （ r = 0.618, P = 0.000 1）. There was no significant correhtion （r= -0.280, P=0.353） between the VEGFR- 3 expression （median 0.65） and Proxl expression （ median 0.65） in tumor tissues. Conclusions VEGFR - 3 and Proxl may synergieally involve in the lymphangiogenesis in normal intestinal tissue and mesentery lymph nodes, but they lack of correlation in tumor tissues.