摘要
采用蚕豆根尖微核实验和姊妹染色单体交换实验,研究亚硒酸钠(0.01～10.0 mg·L-1)对蚕豆根尖细胞的遗传损伤效应.结果表明:一定浓度的亚硒酸钠(Na2SeO3)可导致蚕豆根尖细胞有丝分裂指数下降,间期细胞微核频率明显增高,且Na2SeO3的上述效应具有一定的剂量效应关系;同时Na2SeO3可诱导细胞姊妹染色单体交换(SCE)频率显著增高.研究表明,亚硒酸钠对蚕豆根尖细胞具有遗传毒害作用,并有可能对人体产生遗传损伤.
Micronucleus test and sister chromatid exchange assay were adopted to study the genetic damages of Na2SeO3 at 0.01～10.0 mg/L to the root- tip cells of Viola faba. The results indicated that Na2SeO3 at a certain concentration could cause the mitotic index of the root-tip cells of Vicia faba to decline and the micronucleus frequency at the mitosis interphase to significantly increase, and these effects related to its dosage to some extent ; at the same time, Na2SeO3 could make the frequency of sister chromatid exchange significantly rise in the root-tip cells. The study showed that Na2SeO3 was genetically detrimental to the root tips of Vicia faba and probably capable of causing genetic damage to human bodies.
出处
《西北植物学报》
CAS
CSCD
北大核心
2005年第10期2107-2111,共5页
Acta Botanica Boreali-Occidentalia Sinica
基金
山西省教委应用开发项目基金资助(200305)
关键词
亚硒酸钠
蚕豆
姊妹染色单体交换
微核
sodium selenite
Vicia faba
sister chromatid exchange
micronucleus
