Ad-huVEGF_(121)基因转染对兔股骨头Ⅰ型胶原表达及ALP活性的影响

Enhancement of AdhuVEGF_(121) gene transfection to ALP activity and collagen Ⅰ expression in femoral head of rabbits

目的观察VEGF在坏死股骨头内诱导成骨的作用。方法重组腺病毒Ad-hVEGF121注射入坏死的股骨头,应用酶学和免疫组织化学技术检测血清碱性磷酸酶(ALP)的活性和Ⅰ型胶原(collagenⅠ)的表达。结果基因转染组ALP活性和collagenⅠ表达在各个时相点显著高于空病毒对照组。结论VEGF具有在坏死股骨头内诱导成骨的功能。

Objective To observe the effects of inducing ossification in necrotic femoral head by VEGF. Methods Healthy rabbits were Lesioned into necrotic femoral head by liquid nitrogen and randomly assigned for Ad-huVEGF121 gene transfeetion into the necrotic femoral head and Ad-Traek-CMV transfection. The alkaline phosphatase （ALP） activity before transfection and day 1, week 1, 2, 4, 6, 8, 12 after transfection and the expression of collagen I at week 2, 4, 8, 12 was examined by enzymology and immunohistochemical techniques. Results The ALP activity of the Ad-huVEGF121 group was significantly higher than Ad-Track-CMV group （ P 〈 0.01 ） , and the ALP activity reached the peak at week 2 and then declined in two groups. In the tissues of rabbits with Ad-huVEGF121 gene transfection, collagen Ⅰ expressed in a few cells at week 2, much more at week 4, and there appeared bone trabecula till week 6 to 8, dense bone trabecula at week 12. In rab- bits with Ad-Track-CMV transfection, the collagen Ⅰ expression was less than that with Ad-huVEGF121 gene transfection （P 〈0.01 ） , and there appeared the bone trabecula at week 12. Conclusion VEGF121 can induce ossification in necrotic femoral head.