左向右分流先天性心脏病儿童AT2受体的表达

Expression of angiotensin Ⅱ receptors type 2 in children with left-to-right shunt

目的探讨未发生梗阻性肺动脉高压的左向右分流先天性心脏病儿童骨骼肌血管是否有AT2受体表达,及肺循环AngⅡ受体的表达。方法20例左向右分流先心病患儿术中取肺、骨骼肌活检。用RT-PCR和免疫组化的方法检测这些骨骼肌中的AT2受体;并对肺组织中AT1和AT2受体的mRNA作半定量分析。结果RT-PCR在这些骨骼肌中均检出AT2受体的mRNA;这些骨骼肌血管的AT2受体免疫组化染色均为阳性。这些患儿肺组织中AT2受体的mRNA水平比AT1受体高(P<0.05)。结论左向右分流先心病儿童骨骼肌血管有AT2受体表达;肺循环AT2受体表达水平比AT1受体高。

AIM： To explore whether AT2 receptor is expressed in skeletal muscular vasculature, and the expression of angiotensin Ⅱ receptors in pulmonary circulation of children with left - to - right shunt but without obstructive pulmonary hypertension. METHODS： Lung and skeletal muscular tissue were obtained from 20 children with left - to - right shunt by biopsy during operation. These skeletal muscular tissues were detected by reverse transcriptase/polymerase chain reaction （RT- PCR ） and immunohistochemistry techniques for AT2 receptors, mRNA of AT1 and AT2 receptors in lung tissues were detected by RT- PCR and analyzed semi - quantitatively. RESULTS： In all of the skeletal muscular tissues, mRNA of AT2 receptor was found by RT - PCR, and the results of immunohistochemistry staining for AT2 receptor of vessels were positive. In the lung tissues, the level of mRNA of AT2 receptor was different to AT1 receptor, and the former was higher than the latter （P 〈 0.05）. CONCLUSION： In children with left- to - right shunt, AT2 receptor is expressed in skeletal muscular vasculature, and the level of AT2 receptor expression is higher than that of AT1 receptor in pulmonary circulation.