小檗碱对人高转移肺癌系(PG)细胞增殖的影响及机制研究

Effects of berberine on the proliferation of human pulmonary carcinoma cells

目的通过离体实验初探小檗碱对高转移性人肺癌细胞株(PG)增殖能力的影响,并探讨其作用机制。方法用MTT法测定小檗碱对PG细胞增殖能力的影响。用流式细胞术测定小檗碱对PG细胞细胞周期及凋亡的影响。用激光共聚焦显微镜观察小檗碱诱导PG细胞氧自由基的产生。结果小檗碱能够抑制PG细胞增殖,且随药物浓度增加抑制效应增强,呈浓度依赖性(P<0.05,P<0.01)。小檗碱对PG细胞细胞周期具有阻滞作用,40mg/L的小檗碱能引起PG细胞的凋亡。小檗碱作用6h、12h只有在终浓度大于或等于10mg/L时开始产生氧自由基,低浓度的小檗碱作用24h后就能诱导氧自由基的产生。结论小檗碱对PG细胞增殖具有抑制作用,后者可能与调节细胞内活性氧自由基产生从而影响细胞周期进程有关。

AIM： To study the effect of berberine on the proliferation of human pulmonary carcinoma cells （PG cells）. METHODS： The proliferation of PG cells was determined by using MTT assay. Cell cycle and apoptosis of PG cells were determined by using flow cytometry. Confocal scanning imaging system was used to assay the ROS - releasing level of PG cells. RESULTS： Berberine was shown to inhihit proliferation of PG cells directly and in a concentration - dependent manner （ P 〈 0.05, P 〈 0.01 ）. Berberine blocked the cell cycle of PG cells, and beberine at concentration of 40 mg/L induced the apoptosis of PG cells. After 6 or 12 h incubation, berberine began to induce ROS- releasing in PG cells at 10 mg/L. While incubated with berberine for 24 h, PG cells were induced to release ROS at lower concentration. CONCLUSION： Berberine has an inhibitory effect on the proliferation of PG cells, and these effect may be carried out by regulating the production of intracellular ROS and the process of cell cycle.